HLA-DRB1 antibody

Catalog No. ABIN6296129

The Mouse Monoclonal anti-HLA-DRB1 antibody has been validated for WB, FACS, IF and IHC. It is suitable to detect HLA-DRB1 in samples from Human and Monkey.

Quick Overview for HLA-DRB1 antibody (ABIN6296129)

Target: HLA-DRB1 (Major Histocompatibility Complex, Class II, DR beta 1 (HLA-DRB1))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-DRB1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-HLA-DRB1 Antibody

Purpose: Mouse anti-Human/Monkey HLA-DRB1 Antibody Cocktail (MHC II) [Sodium Azide Free]

Specificity: Cell surface

Purification: Activated human peripheral blood mononuclear cells (LN-3 & HLA-DRB/1067) were used as the immunogen for the HLA-DRB1 antibody cocktail.

Immunogen: Activated human peripheral blood mononuclear cells (LN-3 & HLA-DRB/1067) were used as the immunogen for the HLA-DRB1 antibody cocktail.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for HLA-DRB1

Target: HLA-DRB1 (Major Histocompatibility Complex, Class II, DR beta 1 (HLA-DRB1))

Alternative Name: HLA class II histocompatibility antigen, DRB1-15 beta chain

Background:

Target Description: This mAb reacts with the beta-chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 Molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma.

Gene Symbol: HLA-DRB1///HLA-DRB2

Gene ID: 3123

UniProt: P01911

Pathways: TCR Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response